Chromosome jumping is a technique of molecular biology that is used as a tool in the physical mapping of genomes. It is related to several other tools used for the same purpose, including chromosome walking. Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot be easily mapped by chromosome walking, and is useful in moving along a chromosome rapidly in search of a particular gene. In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes, and circularised (the beginning and end of each fragment is joined together to form a circular loop) Continua »